White prairie clover [Dalea candida (Michx.) Willd.] is native to the dry prairies and hillsides of the Northern Great Plains. A study was initiated in 2012 with six white prairie clover populations collected from the Canadian Prairies. Plant growth characteristics, forage biomass, seed yield, and forage nutritive values were evaluated using a randomized complete block design in a field near Swift Current, SK. Three populations from Argyle, Carlowrie, and Big Grass Marsh (NCP588) in Manitoba displayed erect-type growth while those from Douglas Provincial Park and Stewart Valley in Saskatchewan and Writing on Stone Provincial Park in Alberta exhibited prostrate growth. The populations did not differ for mean biomass yield (79–104 g plant^-1, p = 0.54) and mean seed yield (6.6–9.1 g plant^-1, p = 0.69); however, they differed for bloom stage nutritional parameters such as acid detergent fibre (25%–30%, p = 0.04), neutral detergent fibre (34%–41%, p < 0.01), crude proteins (15%–18%, p < 0.01), phosphorus (0.24%–0.29%, p = 0.02), and iron content (144–360 ppm, p = 0.01). To our knowledge, this is the first report of comparative phenotypic, nutritional, and propagation study of native white prairie clover populations of Canada. The constraints and opportunities for successful domestication of white prairie clover as a forage crop are discussed.