The strawberry production season can be extended in Ontario if plantings are protected from adverse weather conditions. At present, dayneutral cultivars developed in California are used for production, but are not well adapted to Ontario conditions. The objectives of this study were to determine suitable dayneutral cultivars for production throughout Ontario and to investigate how they react to high tunnel environments. Two sets of trials were run. The first set, planted in 2005 and harvested in 2005-2006, compared six dayneutral cultivars of strawberries in four environments. The second set, planted in 2010 and harvested in 2010-2011, compared five dayneutral cultivars grown in four environments. For the summer crops, high tunnels had higher yields in the cooler environment at New Liskeard and low yields in the warmer environment of Cedar Springs, when compared with outside plantings. In the spring, this trend was reversed. ‘Seascape’ and ‘Tribute’ performed consistently in all environments. ‘Albion’ and ‘Monterey’, although medium yielding with lower winter survival, had large fruit size and exceptional fruit quality. ‘Portola’, had large fruits and performed well, so could be of interest to farmers in Ontario. All the European cultivars tested had medium to small berries.