Li, G., Zhang, Z.-S., Gao, H.-Y., Liu, P., Dong, S.-T., Zhang, J.-W. and Zhao, B. 2012. Effects of nitrogen on photosynthetic characteristics of leaves from two different stay-green corn (Zea maysL.) varieties at the grain-filling stage. Can. J. Plant Sci. 92: 671-680. The effects of nitrogen on the photosynthetic characteristics of two different stay-green corn (Zea mays L.) at the grain-filling stage were studied. Using a stay-green inbred line (Q319) and a non-stay-green inbred line (HZ4) as materials, using fast chlorophyll fluorescence-induction kinetics and 820 nm light-absorption curves, we analyzed the influences of nitrogen supplementation on photosystem I (PS I) and photosystem II (PS II). The results show that nitrogen fertilization has significant effects on promoting the net photosynthetic rate (P_n) in leaves at the grain-filling stage and on single-plant grain yield at the harvest stage (P<0.05) in Q319, whereas there is no significant effect in HZ4. Analyses performed using JIP-Test showed that nitrogen fertilization significantly increased electron donor and acceptor performance in the reaction center of PS II (P<0.05). The increased performance of the electron transport chain at a point after the electron acceptor in the PS II reaction center was greater in Q319 than in HZ4, reducing excessive excitation energy production in PS II and significantly improving the coordination between PS II and PS I. Although the performance of the electron transport chain after the electron acceptor in the PS II reaction center was increased in HZ4, the increase was not substantial enough to improve the coordination between PS II and PS I; therefore, P_n and grain yield were still significantly lower than those of Q319.