A total of 320 one-day-old Cobb 500 chicks with an initial weight of 48.3 ± 3.3 g·pen⁻¹ were assigned to four dietary treatments with eight replicates provided in three phases for 46 d. The treatments were fed as mash diets and included (1) negative control (NC) corn-soybean basal diet, (2) positive control (PC) basal diet with 30 ppm avilamycin, (3) basal diet supplemented with 1000 ppm red-osier dogwood extracts (RDE1), and (4) basal diet with 3000 ppm red-osier dogwood extracts (RDE2). Results showed reduced jejunal crypt depth in RDE1 and increased villus:crypt ratio in groups (either RDE1 or RDE2) (P < 0.05). Cationic amino acid (AA) transporter mRNA abundance was decreased (P < 0.05) in RDE1, RDE2, and PC treatments, but peptide and neutral AA transporter mRNA abundance were higher (P < 0.05) in RDE2 compared with NC. Apparent ileal digestibility of crude fat was increased in RDE2 and PC compared with NC, whereas AA digestibility was greater in RDE1, RDE2, and PC (P < 0.05). In conclusion, red-osier dogwood had no effect on growth performance, improved the intestinal health and function of broiler chickens, and had no detrimental effects on meat quality.