Due to the high frequency of post-surgical infection, antibacterial coated suture material has been developed and is suggested to reduce infection during healing. Triclosan is a common antibacterial that is now being incorporated into suture material. When the sutures are placed in the body, the triclosan is slowly released into the tissue over time. Recent studies show a potential for accumulation in fatty body tissues which would amplify the exposure to triclosan. Since exposure time to the drug plays a significant role in the magnitude of its results, the effects on tissue cultures should be investigated. The purpose of this study was to determine if triclosan inhibits tissue proliferation at supraphysiological doses to find out whether or not further study is required. Rat peritoneal tissue was excised from neonatal rats. It was hypothesized that triclosan would have a significantly negative effect on tissue proliferation. However, the results showed that even at high doses, triclosan had no effect on proliferation.