This is the second part of a systematic analysis of the Lycosidae collection of Walter Wittmer from Bhutan, dealing with 16 species from subfamilies Lycosinae (Lycosa and Ovia), Pardosinae (Draposa, Wadicosa, and the Pardosa species-groups nebulosa, paludicola, sutherlandi, taczanowskii, and wagleri) and Hippasinae (Hippasa). Ovia emae sp. nov. is newly described. Descriptions of the yet unknown female of Ovia alboannulata (Yin, Peng, Xie, Bao & Wang, 1997) and the male of Pardosa mukundiTikader & Malhotra, 1980 are provided. All species are illustrated and their affinities are discussed.