In some spiders, nuptial gifts consist of prey or inedible items wrapped in silk by males and offered to females during courtship. Such gifts occur in the Neotropical family Trechaleidae, of which most species are semi-aquatic, associated with watercourses in riparian habitats. Here, we describe the sexual behaviour of the South American species Trechaleoides keyserlingi and report the presence of nuptial gift-giving behaviour for the first time in the genus.