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1 May 2013 Molecular Characterization of Two Sensory Neuron Membrane Proteins from Chilo suppressalis (Lepidoptera: Pyralidae)
Su Liu, Fei Qiao, Qing-Mei Liang, Yuan-Jie Huang, Wen-Wu Zhou, Zhong-Jun Gong, Jiaan Cheng, Zeng-Rong Zhu
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Abstract

Sensory neuron membrane proteins (SNMPs) play an especially important role in insect pheromone communication. However, the SNMPs for the Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), a notorious rice pest, remain uncharacterized. Here we report the cloning and characterization of two novel SNMPs from the C. suppressalis, CsupSNMP1 and CsupSNMP2. The CsupSNMP1 and CsupSNMP2 cDNAs contained open reading frames (ORFs) of 1,572 and 1,569 bp, encoding proteins of 523 and 522 amino acid residues, respectively. The amino acid identity between the two deduced CsupSNMPs was low (30% identity), but they shared a high degree of similarity to previously characterized SNMP1s or SNMP2s from other moth species, which is consistent with phylogenetic analysis in which CsupSNMP1 and CsupSNMP2 are clustered into two distinct groups based on their amino acid sequences. The expression patterns of CsupSNMPs in various adult tissues and in different developmental stages were determined by real-time quantitative polymerase chain reaction. The results showed that both CsupSNMP1 and CsupSNMP2 were abundantly expressed in the male and female antennae, reaching their maximum in the adult stage, suggesting the two genes are involved in the process of olfaction. Low levels of CsupSNMP2 also were expressed in nonolfactory tissues such as legs and wings, implying possible gustatory roles of the protein in the moth.

© 2013 Entomological Society of America
Su Liu, Fei Qiao, Qing-Mei Liang, Yuan-Jie Huang, Wen-Wu Zhou, Zhong-Jun Gong, Jiaan Cheng, and Zeng-Rong Zhu "Molecular Characterization of Two Sensory Neuron Membrane Proteins from Chilo suppressalis (Lepidoptera: Pyralidae)," Annals of the Entomological Society of America 106(3), 378-384, (1 May 2013). https://doi.org/10.1603/AN12099
Received: 6 August 2012; Accepted: 1 February 2013; Published: 1 May 2013
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KEYWORDS
Chilo suppressalis
expression pattern
molecular cloning
real-time quantitative polymerase chain reaction
sensory neuron membrane protein
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