Chrysomelid beetles inhabiting the herb, shrub and tree layers of two mixed forest ecosystems dominated by pine-oak-hawthorn were studied during April–October in 2005 and 2006 in Isparta province, Turkey. The leaf beetles of both sites were investigated in terms of species composition, dominance structure and vegetation preference. Frequency values and host plants were also provided for some species. A total of 127 Chrysomelidae species belonging to ten subfamilies were collected. Species composition similarity between the herb and tree layers was 3% at both sites, while similarity between the shrub and tree layers was 25% at site I and 44% at site II. In terms of vegetation structure, the herb layers of the two sites shared 60% of their chrysomelid species, the shrub layers shared 44% and the tree layers shared 50%, a result also reflected in a PCA analysis. The herb layer was the most diverse vegetation stratum in terms of leaf beetle diversity, and it appears that vegetation cover is the main factor influencing leaf beetle species composition at both forest stands studied.