Discoveries of North American populations of the introduced terrestrial slug, Ambigolimax parvipenis, formerly treated as Ambigolimax nyctelius (Bourguignat, 1861), have increased in frequency over the last fifteen years. Most of these finds are occurring in the western United States of America. In 2015, a single specimen of A. parvipenis was collected in La Jolla, San Diego County, California, U.S.A. Here we report on an established population of A. parvipenis found in a residential backyard in San Diego, San Diego County and confirm through resampling that the single specimen collected in 2015 in La Jolla was not an incidental find.