The present study describes the anatomy of Anemia villosa, Anemia organensis, and a hybrid of the two species (Anemia villosa x Anemia organensis). The aim of the present study was to answer the following question: can the Anemia villosa x Anemia organensis hybrid be distinguished from the parent plants based on its anatomical traits? The results showed that the two species and hybrid can be easily differentiated by their anatomical characteristics and that the hybrid anatomy shares features with both parents. In addition, the hybrid differed from the parents in two variables: leaf thickness and number of trichome cells.