Non-marine calcareous microfossils (ostracods and charophytes) and primitive angiosperm seeds (Carpolithus sp.) from the Piedra Clavada Formation (early Albian to early late Albian) in its type area, at Quebrada Don Nielsen section, Santa Cruz Province, Argentina are described and illustrated for the first time. The microfauna suggests a marshy lagoon paleoenvironment. Affinities with Lower Cretaceous microfossil assemblages from the Golfo San Jorge Basin are included.