The first epidemic of dengue occurred in Burkina Faso in 1925 and the most recent in 2016. However, there is little available information on the bio-ecology of the main dengue vector and the prevalence of the disease in the country. For better understanding the entomological situations of the main dengue vector Aedes aegypti during the last epidemic of dengue, a cross-sectional study was conducted in Bobo-Dioulasso from November to December 2016. The goals of this study included: i) searching for water containers, and classifying them according to their type and location (indoors or outdoors); ii) examining the containers for the presence of Aedes larvae and iii) evaluating the susceptibility to insecticides mainly used in public health. Our results showed that discarded artefacts and automobile parts filled with rain water were breeding grounds for Ae. aegypti while indoor containers were mud pots (domestic purposes). The other breeding sites were metal drums, paraffin tins and mud pots. Moreover, the adults of Ae. aegypti population from these breeding sites were resistant to organochlorines, carbamates and pyrethroids, but fully susceptible to organophosphates. Our data could provide useful information for national programmes in strategy development.