The Aphidiinae (Hymenoptera: Braconidae) are solitary endoparasitoids and koinobionts which are frequently used as biological control agents against aphids. Six aphidiine genera and 15 parasitoid species from 18 aphid taxa occurring on 42 plant taxa belonging to 18 botanic families were identified and 49 parasitoid-aphid associations are reported. In addition, this survey exhibits two species which are Aphidius platensis (Brethes, 1913) and Praon barbatum (Mackauer, 1967) and they are reported for the first time in Tunisia.