The polyandrous mating behaviour of the honeybee queen increases the genetic variability amongst her worker offspring and the genetic variability within the honeybee colony can affect their polyethism. In this study, we intend to understand whether there is genetic variability in the task of the queen retinue. Microsatellite DNA analyses revealed a total of 13 and 12 subfamilies in two colonies, respectively. It shows that the subfamily proportion of the queen retinue workers significantly deviated from random distribution, which suggests that they might have a genetic preference in the task of the queen retinue.