Eukaryotic Expression and Biochemical Characterization of Intestinal Mucin SeIIM8 from Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)

X.N. Li; R.J. Li; D.Q. Xu; W. Guo; W.M. Sun; D. Zhao; D.Q. Liu

doi:10.4001/003.020.0212

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X.N. Li,¹ R.J. Li,¹ D.Q. Xu,² W. Guo,^1,2,3,* W.M. Sun,¹ D. Zhao,¹ D.Q. Liu¹

¹1College of Plant Protection, Agricultural University of Hebei/Biological Control Centre of Plant Di
²2College of Life Sciences, Agricultural University of Hebei, Baoding, Hebei 071001, China
³3National Research Center for Agro-engineering in North Mountainous Areas, Baoding, Hebei 071001, Ch

^*To whom correspondence should be addressed. E-mail: guowei_hbau@yahoo.com.cn

Abstract

The invertebrate intestinal mucin is a major protein constituent of the peritrophic membrane. A SeIIM8 identified from Spodoptera exigua larvae contains a 16-amino acid signal leading sequence and six chitin-binding domains with one intervening mucin-like domain and one Aps-Lys-rich domain. Prediction of O-glycosylation indicated that the amino acid residues from 97 to 244 were potential O-glycosylation sites. Recombinant SeIIM8 expressed in baculorvirus-infected insect cell lines binds chitin in vitro.

Citation Download Citation

X.N. Li, R.J. Li, D.Q. Xu, W. Guo, W.M. Sun, D. Zhao, and D.Q. Liu "Eukaryotic Expression and Biochemical Characterization of Intestinal Mucin SeIIM8 from Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)," African Entomology 20(2), 252-258, (1 September 2012). https://doi.org/10.4001/003.020.0212

Accepted: 1 May 2012; Published: 1 September 2012